Colon Cancer Research Today is a free monthly online journal that collates and summarizes the latest research about Colon Cancer, including details on causes, treatment, symptoms.

Cytogenetic characterization and cell cycle analysis of three human colon adenocarcinoma cell lines: comparison between two- and three-dimensional cell culture systems.

Roncoroni L, Elli L, Bardella MT, Dogliotti E, Grimoldi MG, Viaggi S, Erba E, Zunino A, Doneda L

Department of Biology and Genetic for Health Sciences, University of Milan, Via Viotti, Milan, Italy. leda.roncoroni@unimi.it

The aim of the study was to investigate whether changes in the pattern of gene copy number and cell cycle were present passing from the two- to the three-dimensional cell culture system. We used three human colon adenocarcinoma cell lines grown two- and three-dimensionally. We analyzed morphology, karyotype, chromosomal gain and losses, and cell cycle. In three-dimensional cell cultures the growth is delayed and arrested in G1 phase without specific rearrangements in the three-dimensional cultures compared to the two-dimensional cultures. These data suggest that the differences between the two- and three-dimensional cell culture systems do not involve chromosomal rearrangements.

Published 21 December 2009 in Cancer Invest, 28(1): 7-12.
Full-text of this article is available online (may require subscription).

© 2004-2010 Colon Cancer Research Today. All Rights Reserved.